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KMID : 0380020060210030171
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 3 p.171 ~ p.176
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In Vitro Study of Fluorescence Detection for Protoporphyrin 9 Induced from 5-Aminolevulinic Acid in Cancerous and Normal Cells
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Kim Myung-Hwa
Kim Hyun-Jeong
Lee In-Seon
Kim Kyung-Chan
Lee Chang-Seop
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Abstract
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To clarify the usefulness of fluorescent diagnosis for cancer, We investigated the optimal method of administrating 5-aminolevulinic acid (5-ALA) by analyzing fluorescence signal of Protoporphyrin IX (PpIX) in the cultured normal and cancer cells. 5-ALA was injected as a photosensitizer to the cervico-uterine cancer cell line (HeLa) and in normal liver cells (Chang). Chang and HeLa cells were incubated with various concentrations of 5-ALA (0-800 ㎍/㎖). The accumulation of PpIX induced by 5-ALA was in HeLa and Chang cells. The cell viability was measured by MTT assay. The optimal concentration of ALA that induced maximum levels of PpIX was 50 ㎍/㎖ in HeLa cell cultured for 24 hours after 5-ALA injection. Fluorescence of PpIX in HeLa cell was excited at a wavelength (λ=410 ㎚) and showed an emission spectrum at 602.3 ㎚, 659.9 ㎚ which could be related to the PpIX generation induced by the applied 5-ALA. The experimental results showed that fluorescence signal of PpIX was proportional to the concentration of 5-ALA in cancer cells, but measured with low concentration in normal cells.
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KEYWORD
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5-aminolevulinic acid, Protoporphyrin 9, Photosensitizer
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